Antelope Hill Provincial Park, located in the Northern Fescue Natural Subregion, is comprised of hummocky terrain dominated by scenic grasslands. Localized depressions and wetlands are often ringed with willow shrublands and sporadic, small aspen stands. Over 20 species of uncommon or rare vascular plants are present. The park supports several fescue grassland communities that are at risk in the province. Signs of historical cultivation are evident in a few areas of the park.

The park and surrounding landscape provide habitat for a diversity of birds including the White-faced Ibis, the American White Pelican, and the elusive Sprague’s Pipit. This hard-to-spot sparrow-sized, ground-feeding bird is listed as “Threatened” in Canada under the federal Species at Risk Act. Nearby Dowling Lake is a nationally identified Important Bird Area.

Many large and small mammals frequent the park and some make their home in Antelope Hill Provincial Park. Deer and moose are often observed. It's not uncommon to see thirteen-lined ground squirrels, red foxes and porcupines.